There are various methods for protease detection, and the following are three of them for you:
The first method is the phenol reagent method. This method uses casein solution as the substrate, adds enzyme solution to a specific buffer, and then adds folinol reagent for color reaction. By measuring the optical density value, the activity of protease can be determined.
The second method is ultraviolet spectrophotometry. This method uses a UV spectrophotometer to quantitatively determine the tyrosine produced by protease hydrolysis of casein. By comparing the absorbance values of the sample and the blank sample, the activity of the protease can be calculated.
The third method is high-performance liquid chromatography. This method uses a high-performance liquid chromatography to separate and quantitatively determine the tyrosine produced by protease hydrolysis of casein. By comparing the content of tyrosine in the sample and standard, the activity of protease can be calculated.
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