The operation steps of using protease inhibitor mixture for culture medium mainly include the following points:
Storage conditions: The protease inhibitor mixture should be stored in an environment of -20 ℃ and its shelf life should be one year.
Usage: When using, the protease inhibitor mixture should be added to the culture medium in a specific ratio to ensure its effectiveness. The specific proportions and usage methods may vary depending on different experimental requirements and types of culture media.
Caution: Before use, ensure that the protease inhibitor mixture is completely dissolved in the culture medium to avoid precipitation or crystallization, which may affect its protease inhibition effect.
In addition, the use of protease inhibitor mixtures also involves basic laboratory operating procedures, such as aseptic handling, proper storage and disposal, to ensure the accuracy and safety of experiments. The specific operation steps may vary depending on the specific requirements of the experiment, so in actual operation, specific experimental guidelines or operation manuals should be referred to.
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