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SKU: 2022-001-1-2-1-1-1-1

DIFF HTLV PROTEASE INHIBITOR SCREENING SYSTEM

$8,900.00

Replicable with One-time Purchase; Forward Screening

Use of DIFF Biotech products falls under our material transfer agreement and is for non-profit research use only. For commercial use, please contact us for licensing.

HIV/HTLV protease is an aspartic acid protease that is active only as a homodimer. It plays a very critical role in the maturation and replication of the HIV/HTLV virus, and is able to cleave polyproteins expressed by the gag gene and gag-pol gene of HIV (and the gag gene and gag-pro-pol gene of HTLV) into proteins required by the virus. 

The assay system is based on the co-expression mechanism of a customized plasmid combining recombinant green fluorescent protein (rGFP) and HIV/HTLV protease protease. In this system, a specific cleavage site for the HIV/HTLV protease is embedded in the rGFP sequence. When the HIV/HTLV protease functions normally, it specifically cleaves rGFP, resulting in loss of its fluorescence function and diminished fluorescence intensity. On the contrary, when the inhibitor effectively inhibits the activity of HIV/HTLV protease, rGFP is unable to be cleaved, maintains its integrity and emits fluorescence normally with enhanced fluorescence intensity. By measuring the change in fluorescence intensity, the efficacy of the inhibitor on the HIV/HTLV protease can be visually assessed.

Before You Purchase

 

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

An order for materials must have a completed Material Transfer Agreement (MTA) so that the technology can be transferred from one institution to another. The MTA will be sent to you through email after you place an order. We will only ship your order after we received the approved MTA.

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